FastQCFastQC Report
Fri 21 Jul 2017
HMN35AFXX_n01_mah137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN35AFXX_n01_mah137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544403
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11250.038.4074174
GCAGCGT10900.034.3599851
CAGCGTC13350.030.747512
AGCGTCA14300.029.7120323
CGTCAGA16700.026.304485
TATACCG11150.021.3135325
AGATGTG23050.019.989299
CAGATGT22850.019.5341198
GTCAGAT23350.019.1214666
CGGCCCG3451.8653773E-618.7806999
TCAGATG25750.017.334167
GTATTAG18550.016.6955491
GACCGCG3103.2768017E-416.2564137
CTATACT18100.015.9146764
CTAATAC20250.015.29184153
GTATATA46700.015.1142051
GTATTAA26300.015.06201651
ACCGTCC10350.014.607218
GGCGCGC3951.242392E-414.5807968
TATACGG13900.014.5063995
Produced by FastQC (version 0.11.2)