FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19399482
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10645165.487342393987634No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG704280.3630406213939114No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG406310.20944373669358798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG874550.068.073751
CGGCCGA943800.067.2807546
GTCGCGG922650.066.913052
CGCGGCC951850.066.677684
TCGCGGC934450.066.532833
GGCCGAG955500.066.5207757
GCGGCCG959800.066.427835
CCGAGGA950400.066.052829
AGGATCA305700.065.0923412
AGGATCT231700.064.999112
GAGGATC966200.064.6913211
CGAGGAT974050.064.24219510
AGGATCC269800.061.7893812
GCCGAGG1039600.061.23888
GGATCAC70450.059.30617513
GGATCCA96850.059.1553413
GGATCGT53150.059.08893613
GGATCAT86050.058.37914713
GGATCTC71500.058.3375313
GGATCAG77750.058.0512913
Produced by FastQC (version 0.11.5)