FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_9_nlp7_DAM_NLP7_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_9_nlp7_DAM_NLP7_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16274549
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT220520.13549991462128996No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG212170.13036920408670002No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3436600.068.821511
CGGCCGA3460350.068.81996
AGGATCA1123050.068.72497612
AGGATCT866450.068.6091212
GCGGCCG3472800.068.567935
GGCCGAG3487300.068.286397
GGATCTA82050.068.1256113
CGCGGCC3490900.068.09794
CCGAGGA3419600.068.045749
GGATCCA296100.068.0285113
GGATCTC255700.067.8945613
GCCGAGG3509700.067.8276758
GTCGCGG3491500.067.824362
GAGGATC3416700.067.80131511
GGATCAA331700.067.7338813
GGATCCC181050.067.624713
CGAGGAT3436000.067.5150110
TCGCGGC3511050.067.4472963
GGATCAT317150.067.44218413
GGATCTT264050.067.3249613
Produced by FastQC (version 0.11.5)