FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_8_nlp7_DAM_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_8_nlp7_DAM_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15352979
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT709420.46207319113769385No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG683640.44528166162410565No Hit
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC262910.17124363942658946No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT233250.15192491307387315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3394950.068.9811861
CGGCCGA3431500.068.7810066
GCGGCCG3432650.068.714125
AGGATCT819300.068.695512
AGGATCA1044150.068.58481612
GAGGATC3379850.068.4118811
CGCGGCC3442600.068.390234
GGCCGAG3453650.068.3298657
CCGAGGA3404700.068.150989
GGATCTC249200.068.1068513
GTCGCGG3446150.068.0675352
CGAGGAT3403500.068.01980610
GGATCTA96550.067.9728713
GCCGAGG3474450.067.89568
AGGATCG513050.067.8742312
GGATCCA366000.067.8703913
TCGCGGC3461700.067.7451553
GGATCAA316500.067.6253313
GGATCGT143050.067.5072613
GGATCTT242450.067.2744813
Produced by FastQC (version 0.11.5)