FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_7_nlp7_GR_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_7_nlp7_GR_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370687
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT20690.5581528351412377TruSeq Adapter, Index 19 (97% over 40bp)
TCCTCGGCCGCGACCACGCTGCCCTATAGTGAGTCGTATTAGAGATCGGA9370.2527739035898211No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8230.22202019493534975No Hit
CTAATACGACTCACTATAGGGCAGCGTGGTCGCGGCCGAGGAAGATCGGA7770.20961080372389643No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTAGG150.002223848169.99892447
GGTCGCG94350.066.520931
GTCGCGG96600.064.962772
CACTATA3400.064.85194412
ACTATAG3400.063.82254813
CTATAGG3350.063.73036214
TCGCGGC99450.062.889933
TATAGGG3450.060.86862615
CTAATAC3550.060.148031
ACGACTC3550.060.1399156
CGCGGCC104450.060.080454
GATCGTA1350.059.6287114
CTCACTA3750.058.7990910
TAGGGCA3400.058.67556817
TACGACT3650.058.4922455
CGGCCGA108050.057.9491276
GCGGCCG108650.057.9190335
GATCTGT1150.057.82519514
GGATCTA1700.057.6461713
GACTCAC3900.057.4350138
Produced by FastQC (version 0.11.5)