FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_6_col_DAM_NLP7_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_6_col_DAM_NLP7_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9028044
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT347260.3846458878578793No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG342470.3793401981647409No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT212120.2349567636134693TruSeq Adapter, Index 18 (97% over 40bp)
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC185800.20580316179229963No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT169970.1882689096331387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2026600.068.883581
AGGATCT523200.067.88061512
CGGCCGA2071100.067.825916
CGCGGCC2085200.067.4042054
GTCGCGG2074450.067.335052
GCGGCCG2091250.067.30465
TCGCGGC2076800.067.2356343
GGATCAA205500.067.1909913
GGATCTA72900.067.1685613
GGCCGAG2092400.067.1144567
GGATCCA247800.066.9362313
GGATCTT157250.066.68481413
GGATCTG148400.066.6521413
AGGATCA619450.066.45853412
GGATCAT176050.066.4425513
GGATCAC115050.066.2289213
GATCCAT107950.066.1108714
GGATCTC155800.066.0024713
GGATCCC89100.065.71947513
GATCACA28500.065.5804914
Produced by FastQC (version 0.11.5)