FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_5_col_DAM_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_5_col_DAM_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14319301
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT699060.48819422121233436No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG671420.4688916030188904No Hit
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC336000.2346483253616919No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT274400.19162946571204836No Hit
GGTCGCGGCCGAGGATCAAGTTCACCACCGCATGTCGGTACGCTCCAGGC184390.1287702521233404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2792800.069.503081
CGCGGCC2848300.068.465684
GTCGCGG2838000.068.4491962
TCGCGGC2843850.068.305453
GCGGCCG2860200.068.27925
CGGCCGA2863200.068.243826
AGGATCT709950.068.1825712
AGGATCA858100.067.7216612
GGCCGAG2895900.067.4879467
GGATCTT247300.066.95811513
GGATCTA123750.066.8615913
GGATCAA307400.066.7560113
GGATCCA361900.066.62572513
GGATCTG154750.066.5631613
CCGAGGA2874300.066.453329
GGATCTC200850.066.21953613
GCCGAGG2950400.066.218968
GGATCAT233800.065.8841913
GGATCCG250200.065.5385313
GGATCAC158800.065.46065513
Produced by FastQC (version 0.11.5)