FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_3_col_DAM_NLP7_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_3_col_DAM_NLP7_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14445332
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCAAAGGCACGCGCATCGGCGGTGAGGTTGCCGAG481110.33305568885505715No Hit
GGTCGCGGCCGAGGATCCTCCGCCGGCAACTGAAAGTTGACCCGCTTCAT392470.2716933054913518No Hit
GGTCGCGGCCGAGGATCCGCTACGAGCTTCCAGACGGGCACGTAGAGGTT291940.2020998894314094No Hit
GGTCGCGGCCGAGGATCTTGTAGCGGCTAATCAAGGCTTCACCCTCGGAT259340.17953204536939685No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT212600.14717557201177517No Hit
GGTCGCGGCCGAGGATCATCCGTGTTTCAAACCCGGCAGCTTAGTTGCCG201860.13974064424410598No Hit
GGTCGCGGCCGAGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTG197340.1366116057422564No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG193240.13377331860562292No Hit
GGTCGCGGCCGAGGATCACATGGTTCTGCTGGAGTTCGTAACCGCAGCGG181620.12572919750131045No Hit
GGTCGCGGCCGAGGATCTTAGCTACTAGAGAAAGAGGAGAAATACTAGAT163500.11318535288770103No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT160210.11090780052684147TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2770200.068.800031
CGGCCGA2798200.068.599776
AGGATCT706750.068.34486412
GTCGCGG2807200.067.985592
GGCCGAG2824100.067.974737
AGGATCA929550.067.97060412
GGATCCG180300.067.6355513
TCGCGGC2822250.067.607933
GGATCTT248650.067.5827513
GGATCTA67000.067.5485913
GGATCAA318850.067.53400413
GGATCTC216050.067.42802413
GGATCGT105500.067.1838913
GGATCCA214350.066.7054313
CCGAGGA2829150.066.5607159
CGCGGCC2889650.066.3735664
GGATCAT219500.066.0333513
GGATCAC209350.065.9913213
GCCGAGG2915400.065.830798
GGATCTG190000.065.4905513
Produced by FastQC (version 0.11.5)