FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_2_col_DAM_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_2_col_DAM_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17533948
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT861580.49137821099959916No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG858200.48945052192466865No Hit
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC505830.2884860842521034No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT437260.24937909020832047No Hit
GGTCGCGGCCGAGGATCAAGTTCACCACCGCATGTCGGTACGCTCCAGGC242870.13851415551135432No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC195630.11157213423924833TruSeq Adapter, Index 11 (100% over 50bp)
GGTCGCGGCCGAGGATCCGTGGTTTCGCGTATCGGCATGCTCGGGAGCTT182930.1043290421529709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3717900.069.620131
CGCGGCC3793650.068.508084
TCGCGGC3776550.068.494813
GTCGCGG3779800.068.485732
GCGGCCG3810550.068.351815
CGGCCGA3823550.068.145046
AGGATCT1043850.067.6599212
AGGATCA1183500.067.5815912
GGCCGAG3869050.067.355387
GGATCCA477800.067.2271713
GGATCTA175900.066.9192713
GGATCTC276000.066.75703413
GGATCCG315300.066.6621513
GGATCAA434500.066.6443713
GGATCTT411850.066.6126113
CCGAGGA3840750.066.544679
GGATCAT342450.066.52843513
GCCGAGG3926200.066.360618
GGATCAG217000.066.1969313
GATCCAT246050.066.1772914
Produced by FastQC (version 0.11.5)