FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_28_col_input_h3k29ac_4K_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_28_col_input_h3k29ac_4K_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10063913
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2219262.205166121765957No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2125372.1118723899938323No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1111681.1046200419260381No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC406040.40346135742628136TruSeq Adapter, Index 10 (97% over 36bp)
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG376570.37417851287068954No Hit
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231430.2299602550220774No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG175900.17478290998739754No Hit
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109860.10916231092220294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG169650.049.443211
CGTATGC76750.045.96444744
GTATGCC79800.045.91853345
TATGCCG79950.044.6066846
TCGTATG82400.043.40738343
ATGCCGT73050.043.26251247
CTCGTAT83300.043.1482542
TGGGGGG409950.039.863341
GCAATCT128350.038.25694737
GGCAATC173900.036.24639536
CGGCAAT219350.036.20324735
ATCTCGT116200.035.35915440
TCTCGTA104750.034.7471541
AATCTCG129200.034.3476239
TGCCGTC80950.034.19841448
GCCGTCT73250.032.5377949
TCGGCAA253400.031.72525234
CTCGGCA260000.031.1758333
ACTCGGC262000.030.85800632
TGAAAAA148450.030.51115862