FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_27_col_input_h3k29ac_4N_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_27_col_input_h3k29ac_4N_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6748196
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2813224.168847496427194No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2237173.315211947015172No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC1490672.208990373130834TruSeq Adapter, Index 10 (97% over 36bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1376072.039167208539882No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480880.7126052651701285No Hit
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288790.4279514110141437No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208610.30913447090155655No Hit
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142500.21116754759346054No Hit
TAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94940.14068945241068873No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCGCGTATGC82870.12280319066014088TruSeq Adapter, Index 10 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATG75180.11140755247772886TruSeq Adapter, Index 10 (97% over 36bp)
TTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72890.10801405294096376No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC221150.055.32723245
CGTATGC218900.055.14445544
TATGCCG221050.054.81393446
TCGTATG212400.054.75581743
ATGCCGT216600.054.71204447
CTCGTAT212000.054.34695442
AGGGGGG194750.053.5632931
GCCGTCT201750.052.44352749
TGCCGTC216700.052.13650548
CTGCTTG168950.051.8935857
TGCTTGA178050.051.85574758
TCTCGTA222650.051.54302641
ATCTCGT224300.051.03866240
GCTTGAA190800.050.46375359
AGATCTC232700.050.32465438
TGAAAAA241800.050.1705962
GATCTCG231750.049.88119539
CCGTCTT217500.049.4823150
CTTCTGC184450.049.29746254
TCTGCTT185150.048.59996856
Produced by FastQC (version 0.11.5)