FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_26_col_input_h3k29ac_4K_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_26_col_input_h3k29ac_4K_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14470290
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1456051.0062341528746142No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1363780.9424690175525161No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG705810.48776493076503646No Hit
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232590.16073623956396174No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG123900.042.0433431
TGGGGGG298350.036.2223131
AATCGGG95850.032.31455639
CCCGAAT152350.031.61065735
ATCGGGG83000.031.3298140
CCGAATC148000.030.62404836
CGAATCG129800.030.06434237
TCCCGAA169800.028.79474834
GAATCGG111150.028.68497338
ACTCCCG190450.025.98519532
CTCCCGA192400.025.6672633
CACTCCC215750.023.06780831
TCACTCC217950.022.80260730
TTGGGGG87550.021.6724871
GTCACTC235750.021.16985329
ATGGGGG47850.021.1437241
AGTCACT245750.020.19434428
GTAAAAA55000.018.39249863
GGTGTAA30100.017.79205160
CGGGGTT66700.017.31544542
Produced by FastQC (version 0.11.5)