FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_25_col_input_h3k29ac_4N_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_25_col_input_h3k29ac_4N_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3622772
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3520379.717337994221No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2466306.807770403436926No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1793654.951043013471453No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC1105633.051889547561922TruSeq Adapter, Index 3 (97% over 36bp)
TTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG569961.573270412822005No Hit
ATGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG343510.9481965743358953No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG293940.8113676488611483No Hit
AAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167690.46287759759653657No Hit
TAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105130.2901921512035535No Hit
TTTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79880.2204941409506312No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATG78820.2175682046786273TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATGTCGTATGC64700.17859252528174557TruSeq Adapter, Index 3 (97% over 36bp)
ATTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47980.13244002106674116No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG229450.058.934661
ATGGGGG49900.055.629711
CTGCTTG142250.052.8745157
GCCGTCT164700.052.38025349
TATGCCG179650.051.91755746
TGCCGTC173700.051.9027448
ATGCCGT177700.051.83662447
GCTTGAA153750.051.79221359
GTATGCC181450.051.71185345
TGCTTGA150250.051.6912558
CGTATGC182550.051.4194244
CTCGTAT169150.051.29260342
CTTCTGC150000.051.28456554
TCGTATG180700.051.19048743
TCTCGTA166300.051.1404241
CCGTCTT170850.050.6586250
ATCTCGT168450.050.5084740
CATCTCG168900.050.2709839
TCTGCTT151800.049.94004456
CGTCTTC164700.049.87280351
Produced by FastQC (version 0.11.5)