FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_24_col_chip_h3k29ac_4K_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_24_col_chip_h3k29ac_4K_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20473811
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC395890.19336409816423528TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG70700.040.19536246
CGTATGC71400.039.70325544
GTATGCC75350.037.9936945
ATGCCGT78800.036.01936747
TGCCGTC84150.032.8978148
GCCGTCT83250.032.53902449
CTCGTAT88150.032.00015342
TCGTATG90900.031.34006143
CCAGTCA108050.028.05024726
CAGTCAC110700.027.4109827
TATCTCG105100.027.1725239
TCCAGTC114000.026.86245325
TCTCGTA107000.026.68995541
ACGTCTG116100.026.07671715
GTCACTA116150.025.94413629
CCGTCTT109550.025.17442150
CTCCAGT122800.025.07977524
CACTATA120500.024.97918731
CGTCTGA124600.024.38076816
ACTCCAG125100.024.28284823
Produced by FastQC (version 0.11.5)