FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_23_col_chip_h3k29ac_4N_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_23_col_chip_h3k29ac_4N_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19563763
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC412490.21084389542032378TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC76850.043.76473244
GTATGCC79050.042.54695545
TCGTATG88450.038.262643
CTCGTAT87700.035.67653742
TATGCCG99550.034.17142546
GTCACTA109000.031.08177429
CTAATCG110200.030.96626533
TCTCGTA106050.028.64547341
AGTCACT120000.028.55329128
CAGTCAC120700.028.3877727
ATGCCGT120800.028.3631147
CCAGTCA122650.027.85025826
CACTAAT131950.026.25974531
ACTAATC138900.024.87028332
TAATCGA140300.024.44717434
CTGCTTG130600.024.3610857
CTCCAGT145650.023.93292624
TCACTAA147350.023.5388330
CCGTCTT144750.023.42885450
TCCAGTC146800.023.31630725
Produced by FastQC (version 0.11.5)