FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_22_col_chip_h3k29ac_4K_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_22_col_chip_h3k29ac_4K_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25193347
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC390870.15514810318771857TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC95950.034.1775744
GTATGCC103000.031.6344245
TATGCCG102350.031.3904846
TCGTATG112750.029.14711843
CTCGTAT109750.028.66805642
GTCACCC126000.027.58257329
AGTCACC133400.026.1309528
TCTCGTA130150.024.28236641
CAGTCAC148600.023.88205727
CCAGTCA150100.023.85296426
ACCCAAC149700.023.49681332
ACTCCAG156050.023.1228123
CACCCAA155800.022.6441531
TCACCCA156100.022.57812130
ATGCCGT146950.021.76810347
CTCCAGT167950.021.38034824
TCCAGTC171200.021.13801625
GAACTCC175700.020.39719621
CCCAACA183450.019.5173933
ATCTCGT174200.018.56395140
Produced by FastQC (version 0.11.5)