FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_21_col_chip_h3k29ac_4N_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_21_col_chip_h3k29ac_4N_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3550808
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG433641.2212431649359807No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG269590.7592356443941772No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC123550.3479489738673564TruSeq Adapter, Index 9 (97% over 36bp)
ATACATATATATGTATACATATATATGTATACATATATATGTATACATAT50490.14219298818747733No Hit
ATATATGTATACATATATATGTATACATATATATGTATACATATATATGT38130.10738400949868311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGGGG41950.047.4888421
CGTATGC24600.045.3832544
GTATGCC26800.045.3142445
TATGCCG27550.042.81030346
CTCGTAT29750.038.46756442
ATGCCGT26800.038.13186647
TCGTATG28100.037.4886843
TCTCGTA33050.034.52073341
TGCCGTC28250.033.3252848
TATCTCG44050.031.70056339
CGCGTAT7800.029.16437342
GCCGTCT26950.028.69944649
ATCTCGT46100.028.31664540
AGGGGGG5050.027.7319151
CTGCTTG20450.026.87019357
CCGTCTT30250.026.84086250
AAAAGGG29100.026.222370
TATCGCG17800.025.56014639
TCGCGTA9200.025.1067241
GCGTATG8300.024.87791343
Produced by FastQC (version 0.11.5)