FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_20_nlp7_DAM_NLP7_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_20_nlp7_DAM_NLP7_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747171
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC171560.19613198370078735No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT134740.15403837423550998No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG1934500.068.8646241
CGGCCGA1952150.068.8603446
GCGGCCG1954100.068.772155
CGCGGCC1958050.068.575814
GGCCGAG1962300.068.500697
CCGAGGA1963750.068.277529
GCCGAGG1971600.068.170268
GTCGCGG1958750.068.117472
TCGCGGC1962550.068.0012363
AGGATCA613900.067.6284812
GAGGATC1995400.067.0826511
CGAGGAT1999700.066.9817810
AGGATCT454500.066.9409312
GGATCGT112300.065.2980613
AGGATCC529250.063.50483712
GGATCCC100100.063.39569513
GGATCCA178600.063.38034413
GGATCCG131850.063.34120613
GGATCTA62950.063.16531413
GGATCAA187700.062.8996313
Produced by FastQC (version 0.11.5)