FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_19_nlp7_DAM_NLP7_R6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_19_nlp7_DAM_NLP7_R6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10069287
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA2352100.069.08346
GCGGCCG2356550.068.938555
GGCCGAG2364950.068.7031567
GGTCGCG2349800.068.620631
CGCGGCC2368700.068.4903644
CCGAGGA2369000.068.471589
GAGGATC2369700.068.3481811
GCCGAGG2381150.068.2260068
GTCGCGG2372100.068.071652
CGAGGAT2384800.067.9763910
AGGATCA749400.067.8289112
TCGCGGC2385450.067.687753
AGGATCT486550.067.6314512
AGGATCG575300.066.8720912
GGATCCA180100.065.6704613
GGATCCC131300.065.632513
AGGATCC607700.065.6049412
GGATCCG164000.065.1809613
GGATCGT146150.065.14268513
GGATCAA224700.062.90118413
Produced by FastQC (version 0.11.5)