FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_18_nlp7_DAM_NLP7_R5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_18_nlp7_DAM_NLP7_R5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12156788
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2990950.068.585491
GCGGCCG3014900.068.2168965
CGGCCGA3025050.067.956166
CGCGGCC3026800.067.922974
GTCGCGG3027000.067.7623442
GGCCGAG3051650.067.333487
TCGCGGC3047350.067.280563
CCGAGGA3066750.066.936979
GCCGAGG3078500.066.734538
GAGGATC3084900.066.4176711
CGAGGAT3095750.066.250210
AGGATCA972200.065.9500212
AGGATCT628000.065.0824812
AGGATCG782150.065.01420612
GGATCCC177950.064.0632113
GGATCCG200000.063.56305713
GGATCCA224000.062.65926413
GGATCGT213600.062.4163913
AGGATCC803650.061.39336412
GGATCAG283100.060.76766613
Produced by FastQC (version 0.11.5)