FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_17_nlp7_DAM_NLP7_R4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_17_nlp7_DAM_NLP7_R4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12071703
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC150300.12450604525310141No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT128270.10625675598546452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA2720450.068.893576
GCGGCCG2721800.068.860695
GGTCGCG2719200.068.463771
GGCCGAG2741200.068.386697
CGCGGCC2738350.068.336854
CCGAGGA2745450.068.181249
GCCGAGG2757700.067.9923258
GTCGCGG2741600.067.970872
GAGGATC2757550.067.78940611
TCGCGGC2757200.067.558653
CGAGGAT2770600.067.5423910
AGGATCA852000.067.0767912
AGGATCT574300.066.4791112
AGGATCG675350.065.7312612
AGGATCC727450.065.0459612
GGATCCG192900.063.9414613
GGATCCC153050.063.91861713
GGATCGT182700.063.66059513
GGATCCA231550.062.29263313
GGATCAA258750.061.08751313
Produced by FastQC (version 0.11.5)