FastQCFastQC Report
Fri 26 Oct 2018
HMN22BGX7_n01_16_nlp7_DAM_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_16_nlp7_DAM_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11462850
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC209480.182746873596008TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA2733350.068.662346
GCGGCCG2736250.068.610345
GGTCGCG2730550.068.281711
GGCCGAG2753050.068.17517
CGCGGCC2757300.068.0075454
CCGAGGA2765400.067.758489
GTCGCGG2762950.067.5931852
GCCGAGG2779900.067.5265358
TCGCGGC2783250.067.0926363
AGGATCA860800.066.8155612
AGGATCT557200.065.9329112
AGGATCC670800.063.99764312
GGATCGT223100.063.25726713
GGATCCC150450.063.2101613
GAGGATC2972450.062.90788311
GGATCCG185650.062.8013713
CGAGGAT2993300.062.51619310
GGATCTA44850.060.48235713
GGATCCA191750.060.47495313
GGATCAG248800.060.07129313
Produced by FastQC (version 0.11.5)