FastQCFastQC Report
Thu 25 Oct 2018
HMN22BGX7_n01_15_nlp7_DAM_R6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_15_nlp7_DAM_R6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11728272
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC367910.3136949756963345No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT278010.23704259246374915No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT135280.1153452102747958No Hit
GGTCGCGGCCGAGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGC130060.11089442673225859No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG118540.10107200787976267No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA118330.10089295336943073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2594900.069.537671
CGCGGCC2627850.069.106294
GCGGCCG2637000.068.9995355
CGGCCGA2640500.068.915736
GTCGCGG2623350.068.910252
TCGCGGC2629100.068.746223
GGCCGAG2655150.068.51947
CCGAGGA2655200.068.436369
GCCGAGG2675250.068.00248
CGAGGAT2679250.067.6943210
GAGGATC2677400.067.6903911
AGGATCA813350.066.92451512
AGGATCT674550.066.7791412
AGGATCC787100.063.90035612
AGGATCG495600.063.84304412
GGATCCA286950.062.87829213
GGATCGG141850.062.8460413
GGATCGT126600.062.8411313
GGATCAC175600.062.7264213
GGATCTC206450.061.57568413
Produced by FastQC (version 0.11.5)