FastQCFastQC Report
Thu 25 Oct 2018
HMN22BGX7_n01_14_nlp7_DAM_R5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_14_nlp7_DAM_R5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11467141
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC895890.7812671004917442No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT818440.713726289752607No Hit
GGTCGCGGCCGAGGATCGTGGTCTGCAGCACGCGCCTAACGGCGTGCCTC214810.18732655332309944No Hit
GGTCGCGGCCGAGGATCCCGCCAATCAGCTTCCTTGCGCCTTACGGGTTT198040.17270215827990604No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG179750.1567522366734655No Hit
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT155230.13536940027161085No Hit
GGTCGCGGCCGAGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGC140960.12292514760217912No Hit
GGTCGCGGCCGAGGATCGGGTTGCGGTTTAAGTTCTTATACTCAATCATA116130.10127197354597803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG2736850.069.624961
GTCGCGG2772400.068.718262
CGCGGCC2780500.068.700314
GCGGCCG2789300.068.598065
TCGCGGC2777400.068.552963
CGGCCGA2799900.068.401476
GGCCGAG2821900.067.858437
CCGAGGA2830100.067.59949
CGAGGAT2837500.067.40642510
GAGGATC2839800.067.3015911
GCCGAGG2863550.066.871388
AGGATCA807050.066.4689612
AGGATCT713600.066.0992712
AGGATCG479800.065.83813512
GGATCCG266000.063.7661813
GGATCGT138150.063.6439913
GGATCCA349600.063.5956913
AGGATCC936500.063.0144812
GGATCGG137650.062.75633213
GGATCAC173250.062.20496713
Produced by FastQC (version 0.11.5)