FastQCFastQC Report
Thu 25 Oct 2018
HMN22BGX7_n01_13_nlp7_DAM_R4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_13_nlp7_DAM_R4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15173444
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3170250.069.657631
CGCGGCC3207400.069.3360444
GCGGCCG3214300.069.286765
CGGCCGA3219200.069.200646
GTCGCGG3196850.069.153872
TCGCGGC3200300.069.108853
GGCCGAG3234300.068.8810657
GCCGAGG3237750.068.8142558
GAGGATC3228700.068.73656511
CCGAGGA3245450.068.5387659
CGAGGAT3241550.068.5267110
AGGATCG481200.067.9804312
AGGATCA1104950.067.6267712
AGGATCT936500.067.4610712
GGATCGT130750.065.0769313
AGGATCC799650.064.395512
GGATCGG140800.063.5640913
GGATCAA414700.063.54582213
GGATCAC209500.063.41995613
GGATCCA307900.063.2273613
Produced by FastQC (version 0.11.5)