FastQCFastQC Report
Thu 25 Oct 2018
HMN22BGX7_n01_12_nlp7_DAM_NLP7_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_12_nlp7_DAM_NLP7_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12170865
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCGA3025300.069.046546
AGGATCA976300.068.8961612
AGGATCT706250.068.8541712
GCGGCCG3034900.068.824695
GAGGATC2996650.068.71940611
AGGATCG580450.068.6230612
GGCCGAG3044600.068.599557
GGTCGCG3029750.068.4605941
CCGAGGA3014550.068.4191069
GGATCCA206400.068.3748513
CGAGGAT3015150.068.33910
GGATCTA43200.068.22034513
CGCGGCC3059050.068.184684
GGATCCG187450.068.1542513
GCCGAGG3069150.068.074458
GGATCTC212500.068.0592113
GGATCAA255250.068.0144913
GGATCGT165900.067.9141313
GGATCCC176100.067.876113
GTCGCGG3067850.067.732352
Produced by FastQC (version 0.11.5)