FastQCFastQC Report
Thu 25 Oct 2018
HMN22BGX7_n01_11_nlp7_DAM_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_11_nlp7_DAM_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14250102
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGCGGCCGAGGATCCATGCTTTCCAACGAAGCACGCCCATCCAACCT497200.3489097832422533No Hit
GGTCGCGGCCGAGGATCCGCCTAGGCTGTCCCGAGTGTGAGCGAGGTGTG483780.3394923067919093No Hit
GGTCGCGGCCGAGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCC239370.1679777450014042No Hit
GGTCGCGGCCGAGGATCCGGTTAAAATTCCGGAACCGGGACGTGGCGGTT207630.14570422022242366No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCG3098000.068.985271
CGGCCGA3131550.068.722196
GCGGCCG3134750.068.627235
CGCGGCC3140450.068.392094
AGGATCA962600.068.2861612
AGGATCT778200.068.2348112
GGCCGAG3157000.068.163697
GTCGCGG3143550.068.067042
CCGAGGA3109150.067.84189
TCGCGGC3154950.067.821723
GAGGATC3091150.067.7833411
GCCGAGG3180050.067.66428
GGATCTA89900.067.588313
CGAGGAT3107950.067.53020510
GGATCCA334450.067.1032913
GGATCAA294950.067.0117413
GGATCTC222450.066.88676513
GGATCAT279050.066.8664313
GGATCGT121200.066.8543813
GGATCAG213850.066.7451113
Produced by FastQC (version 0.11.5)