FastQCFastQC Report
Thu 25 Oct 2018
HMN22BGX7_n01_10_nlp7_GR_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMN22BGX7_n01_10_nlp7_GR_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286732
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT40341.4068886625838761TruSeq Adapter, Index 22 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15090.5262754070002651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA252.834504E-670.0043614
CCTCCCA150.002224185569.9921626
GCCGGCT207.922884E-569.9921551
GGTCGCG79900.068.415361
GTCGCGG82900.065.939532
GATCAGG2000.063.0039214
TCGCGGC87100.062.5593
CGCGGCC90250.060.4530564
TCTCGTA4950.060.09427643
TCGTATG4900.058.5648645
GCGGCCG93650.058.519875
GATCATA1750.058.00361614
CGGCCGA94900.057.7490546
GATCGTG850.057.6506514
GATCTCC2250.057.55914314
CTCGTAT5200.057.2051344
GGCCGAG96200.057.07787
GATCGTT1350.057.0405914
GATCCGT800.056.87854414
GGATCTA1850.056.76029613
Produced by FastQC (version 0.11.5)