FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_wth4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_wth4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3732626
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC242760.6503732224980483TruSeq Adapter, Index 8 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52550.140785602415029No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG42650.040.79852311
GTCACAC43300.039.67803229
CACACGT43900.039.58672712
ACACGTC44600.039.16269713
CGTCTGA44350.039.1359816
AGCACAC45050.038.91800310
ACGTCTG45050.038.62553415
GATCGGA36100.038.472781
CAGTCAC44900.038.4116127
CACGTCT45450.038.38239714
CCAGTCA45850.037.6647226
TCCAGTC45850.037.5207825
GAGCACA46450.037.508229
ATCTCGT38300.037.3931940
AGTCACA46250.037.24234428
GAACTCC46500.037.23284521
CTCGTAT38150.037.07888442
TCACACT46450.036.84519630
AGAGCAC47600.036.7406778
TCGGAAG47700.036.586613
Produced by FastQC (version 0.11.2)