FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_wt_mms_h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_wt_mms_h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3565635
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC486991.3657875806132709TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG73450.042.0802611
ACACGTC74550.041.4593613
CGATCAG73500.041.39375333
ACGTCTG74600.041.25637415
AGTCACG74050.041.11543728
GATCGGA61550.041.080731
CACACGT75100.041.06785212
CACGTCT75100.041.01099414
GTCACGA74550.040.86976229
CGTCTGA75350.040.75813316
CACGATC75250.040.4895831
AGCACAC76750.040.3569310
GATCTCG68750.040.2235939
GAGCACA77400.040.046449
TCGGAAG78200.039.6776853
CAGTCAC77450.039.50932327
TCCAGTC76950.039.5087525
GAACTCC77750.039.4417621
GTCTGAA78000.039.42981717
TCACGAT77350.039.41875530
Produced by FastQC (version 0.11.2)