FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_rrm3-h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_rrm3-h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362527
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC28720.21078481380552458TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG6000.037.76465211
ACACGTC6300.035.9663313
CACACGT6400.035.74809312
AGCACAC6500.034.52123610
ACGTCTG6850.033.4021315
CGTCTGA7000.032.37207816
TAGCTTA7000.031.74116334
AGAGCAC7450.031.5956298
CACGTCT7350.030.53122114
GAACTCC7350.030.52898221
CAGTCAC7300.030.13537827
GAGCACA7750.029.8048619
TATCTCG6800.029.76420839
CTCGTAT6700.029.55174642
TCCAGTC7500.029.33176825
GTCACTA7500.029.0384529
TCTCGTA7050.029.02079241
GTCTGAA7900.028.40563217
TCACTAG7600.028.36690530
ATCTCGT7150.028.30721940
Produced by FastQC (version 0.11.2)