FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_double-h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_double-h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774784
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT15680.20237898562696183TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG2250.034.2193111
GTCACGT2350.032.7631729
AGTCACG2500.031.67730128
CACACGT2550.031.05618312
CACGTGA2500.030.79737731
TCACGTG2500.030.79737730
CACGTCT2700.029.33462314
ACACGTC2750.028.7975513
TCTCGTA2650.027.39389643
GAACTCC3000.027.13277621
GATCGGA2450.026.9486581
CGTCTGA2950.026.84863916
GATCTCG2550.026.74282541
CTCGTAT2800.026.71201144
AGCACAC2950.026.09947410
AGGGGGG2150.025.5907781
GAAACGA3250.025.04402436
TCCAGTC3300.024.66615925
CCCCGCG450.001398453824.44236636
CAGTCAC3150.024.44236627
Produced by FastQC (version 0.11.2)