FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_498ph2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_498ph2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043406
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC125290.411676917243378TruSeq Adapter, Index 7 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54280.17835280603376613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG24650.036.59089715
ACACGTC24650.036.41180813
CACACGT25100.035.8460612
GCACACG25100.035.7584211
CATCTCG22200.035.67314539
AGCACAC25400.035.42268410
GAGCACA25450.035.353099
CACGTCT25950.034.92680414
ATCTCGT22700.034.7904840
CGTCTGA26050.034.5399516
GTCTGAA26350.034.31368317
TCCAGTC26350.034.06265625
GAACTCC26600.033.9084821
AGTCACC26350.033.72815728
AGGGGGG30900.033.2637751
GATCGGA20600.033.228161
CAGTCAC26750.033.1415727
CCAGATC26950.033.14050333
TCTGAAC27400.032.91845318
CTCGTAT25100.032.60326442
Produced by FastQC (version 0.11.2)