FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_498input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_498input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2226147
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC59410.26687366108347743TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG10100.034.8488211
ACACGTC10250.034.33883313
ACTGACC10350.034.2203732
GATCGGA8300.033.941121
CACACGT10400.033.84356312
ACGTCTG10400.033.63204215
CACGTCT10800.032.79378514
CGTCTGA10900.032.69621316
CCAGTCA11050.031.85562926
CGTATGC11050.031.85276444
AGCACAC11550.030.8547810
TCCAGTC11500.030.60910225
TCGGAAG11650.030.600933
CTGACCA11600.030.53283133
GAGCACA12000.029.6977279
CAGTCAC12100.029.45493727
TCGTATG12050.029.02682143
ATCTCGT10550.028.98356240
CTCGTAT10900.028.65835442
AGTCACT12400.028.56361428
Produced by FastQC (version 0.11.2)