FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_492ph2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_492ph2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18373402
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC675660.36773810315585537TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC97400.038.4869629
TCACGCC99000.037.93161830
AGTCACG98950.037.92875728
ACACGTC102450.037.10459513
GCACACG102800.036.97826811
ACGTCTG103200.036.83594515
CACACGT104500.036.41880812
CACGTCT105900.035.95881314
CACGCCA105600.035.62338631
TATCTCG104750.034.67346639
AGCACAC111500.034.17188610
CGTCTGA112050.033.92654416
GAGCACA113450.033.7202649
GAACTCC112600.033.62388221
CAGTCAC113300.033.26108627
TCCAGTC113650.033.042625
GATCGGA95750.032.614431
CCAGTCA116750.032.16532526
CTCGTAT113200.032.0854742
ATCTCGT114550.031.84150940
Produced by FastQC (version 0.11.2)