FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_492input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_492input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7846282
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129020.16443456913733154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG56000.034.977871
GCACACG17800.025.0879611
CACACGT18800.023.63648412
ACACGTC19350.022.73741713
ACGTCTG19750.022.38886815
GATCGGA15800.022.1478441
CGTCTGA20250.021.61892116
CACGTCT20300.021.45702714
CGTATGC20750.020.2503444
TCCAGTC21550.020.0083825
CCGATGT22100.019.80856133
GTCACCG21800.019.67763929
AGCACAC22750.019.53256610
CAGTCAC22050.019.45453627
CACCGAT22300.019.43360931
TATCTCG21500.019.13402639
GAGCACA23800.019.1329869
TCGGAAG24300.018.9277153
AGAGCAC23850.018.9084018
CGATGTA22850.018.8695734
Produced by FastQC (version 0.11.2)