FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_492input-gal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_492input-gal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7251396
Sequences flagged as poor quality0
Sequence length50
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGGC5850.013.16158733
GCACACG5950.012.94038411
CACACGT7950.09.96151512
CATCTCG7600.09.55250739
ACTTAGG7950.09.40816332
CACGTCT8600.09.20880114
ACACGTC8150.09.17722513
TTAGGCA9450.08.84614134
CGTCTGA9250.08.79964316
ACGTCTG9150.08.65538615
TCCAGTC10350.08.5020725
CCAGTCA10950.07.835298526
AGCACAC10050.07.661222510
CAGTCAC11550.07.618685727
GATCGGA7855.456968E-127.56949471
TAGGCAT11150.07.49740135
TGGGGGG8353.6379788E-127.3793385
GAGCACA11700.07.3328849
CGTATGC11300.07.20334744
CTCGTAT10550.06.88137942
Produced by FastQC (version 0.11.2)