FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_492h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_492h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6359775
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG6900.09.88369311
CACACGT7800.09.30734712
AGGGGGG8000.09.0774481
CGTCTGA9300.08.51588216
GATCGGA7850.07.84925031
ACGTCTG8700.07.585985715
ACACGTC8950.07.37402913
CGATCGC3450.00179082397.014233641
CACAGTC11850.06.68329731
CACGTCT10054.7293724E-116.348073514
GTCACAG11953.6379788E-126.075089529
AGCACAC11151.05501385E-105.919063610
ATCTCGT11653.2923708E-105.665026742
GAGCACA13653.6379788E-125.64081679
CCAGTCA13301.0913936E-115.623940526
AGTCACA12607.6397555E-115.587095328
TCCAGTC12709.458745E-115.54318925
GTCTGAA14751.8189894E-125.518483617
GAACTCC13652.1827873E-115.47973721
CAGTCAC12453.2014214E-105.47779527
Produced by FastQC (version 0.11.2)