FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_492h4-gal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_492h4-gal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6818789
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG8350.017.12464711
GTCACAC9650.015.50159429
CACACGT10150.014.52123412
AGGGGGG16000.014.3050591
ACGTCTG11550.012.95192815
ACACGTC11650.012.65163813
TATCTCG11200.012.37456741
CGTCTGA12300.011.44675416
AGCACAC13000.011.33773210
GAACTCC14650.010.81206721
CACGTCT13150.010.539414
GATCGGA11350.010.4706631
ATCTCGT14500.010.3168842
CACACTG15600.010.294209531
GAGCACA15250.09.9535319
CAGTCAC15800.09.88558527
AGTCACA15100.09.76096228
TCCAGTC15650.09.69941125
TCACACT15900.09.68491230
AGAGCAC16150.09.6713468