FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_492h2a-gal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_492h2a-gal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9713452
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG7100.012.08383428
GCACACG8950.010.32339411
GTCACGT8100.010.32041329
CACACGT9250.09.75075712
ACGTCTG9950.09.28615515
ACACGTC10000.08.7995113
CGTTTCG9650.08.4347233
CACGTCT11100.07.927567514
AGCACAC12500.07.215561410
GAACTCC13250.06.9733421
CGTCTGA12950.06.79516316
GTTTCGG12800.06.702717334
GAGCACA14100.06.24075569
GTCTGAA15100.05.8276417
TCGGAAG19800.05.66816043
CAGTCAC14701.8189894E-125.537163727
TCCAGTC16050.05.482673625
CACGTTT17750.05.453217531
CCAGTCA18200.05.197619426
TGAACTC19800.05.110984320
Produced by FastQC (version 0.11.2)