FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_38ph2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_38ph2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4064971
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC147350.36248721085587077TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA20550.033.7344131
ACGTCTG26700.033.69888715
ACACGTC26850.033.51021213
GCACACG27250.033.26050611
CACACGT27250.033.0990512
CACGTCT27450.032.7777514
CGTCTGA27750.032.50306716
GAACTCC28900.031.5141821
AGCACAC28900.031.51379210
GAGCACA29250.030.9862869
CAGTCAC29600.030.62026827
ATCTCGT25950.030.43485840
TCCAGTC29900.029.94516625
TCGGAAG30550.029.5331483
AGAGCAC30700.029.5227628
GTCTGAA30700.029.45146817
TGAACTC31050.029.19033620
AGGGGGG23650.029.0334011
GTCACCT30950.028.99997729
AGTCACC31400.028.79455228
Produced by FastQC (version 0.11.2)