FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_38input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_38input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753383
Sequences flagged as poor quality0
Sequence length50
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC18910.25100115080908386TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC4000.036.84716413
ACGTCTG4100.036.48499715
GCACACG4100.035.94845211
AGCACAC4250.035.19729210
CACACGT4250.035.19729212
CACGTCT4300.034.7880214
CACAGTG4150.034.4551833
GATCTCG3900.034.40760839
CGTCTGA4300.034.27643216
GAGCACA4450.033.615399
GATCGGA3150.032.840311
CGTATGC4100.032.72918744
GTCACAC4250.032.6114229
AGAGCAC4600.032.041018
CTCGTAT3850.031.9975442
ACACAGT4400.031.9975432
ATCTCGT4150.031.80478340
CACACAG4450.031.64011431
TCCAGTC4600.031.56488425
GAACTCC4550.031.42615321
Produced by FastQC (version 0.11.2)