FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_38h4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_38h4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3269336
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT54440.16651699305302362TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13300.034.90059311
CACACGT13800.033.47666512
ACACGTC14150.032.49315313
ACGTCTG14600.031.64281315
CGTCTGA14650.031.38513216
CACGTCT14800.031.0660914
GATCGGA11950.030.9363861
AGCACAC15800.029.23911310
GAGCACA16400.028.5718129
AGTCACA16300.028.34264228
CAGTCAC16050.028.09920927
TCCAGTC16400.027.7678225
AGAGCAC17050.027.3535378
CACATGT16650.027.21833831
GAACTCC16750.027.18759521
CCAGTCA16650.026.95449626
GTCACAT16900.026.815729
CTCGTAT14800.026.7567144
ATCTCGT14650.026.72951142
TCTCGTA15050.026.45802143
Produced by FastQC (version 0.11.2)