FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_245input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_245input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64191402
Sequences flagged as poor quality0
Sequence length50
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG81950.022.17284411
GATCTCG81050.019.51533139
ACGTCTG96150.018.89893215
CGATCTC84450.018.57330938
GATCGGA75450.018.289891
CGTCTGA106100.017.20955316
CACGTCT108050.017.16346414
AGCACAC108000.017.10983510
ACACGTC109100.016.93749613
CACACGT109600.016.75971212
GAGCACA111850.016.6192479
GAACTCC113150.015.94280821
TCCAGTC116000.015.58913525
CACGATC105100.015.300714536
CAGTCAC120600.015.24981127
CGTATGC121400.015.0224744
AGAGCAC125850.014.9278058
TCGGAAG130450.014.3056373
CCAGTCA135100.013.56433626
CTCGTAT128300.013.23710942
Produced by FastQC (version 0.11.2)