FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_245h4gal.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_245h4gal.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5736963
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG5650.012.070168511
AGTCACG6350.010.73969228
CACGTGG6900.010.52137431
TCACGTG6800.09.70554530
GTCACGT7250.09.4065729
CACACGT7250.09.40640612
ACGTCTG8600.08.69729715
ACGTGGC8150.08.63766232
CGTGGCC8100.08.41946133
GGCCTTA9950.07.296293736
ACACGTC9100.07.25236913
CGTCTGA9450.07.21661816
AGCACAC9700.07.03056210
GAGCACA11400.06.75397549
TATCTCG8703.0449883E-96.3216241
CACGTCT10156.002665E-116.285441414
TCCAGTC12601.8189894E-125.936250725
GAACTCC12357.2759576E-125.878236321
GATCGGA8051.0963486E-65.74113371
CAGTCAC12556.730261E-115.609318327
Produced by FastQC (version 0.11.2)