FastQCFastQC Report
Mon 17 Jul 2017
HMMNMAFXX_n01_245h2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMNMAFXX_n01_245h2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4406197
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG4850.022.67919529
CACGGCT5050.022.6522531
AGTCACG5550.021.40425728
CACACGT5850.019.93010312
ACGTCTG5800.019.72352815
TCACGGC5800.019.7230830
ACACGTC6300.018.85570313
GGCTACA6700.018.71498934
GCACACG6150.018.60020611
CACGTCT6750.017.92516914
CATCTCG6300.017.80836739
TCCAGTC7600.015.9205625
CGGCTAC8100.014.66554633
GAACTCC8300.014.57769721
CGTCTGA8150.014.30630616
AGCACAC8050.014.21009410
ACGGCTA8300.014.0472832
GAGCACA9150.012.98261459
CAGTCAC9250.012.60501527
CTCGTAT9350.011.99922142