FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32914611
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6260601.9020732160559333No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG20800.034.98530670
GCGATCT114650.020.51307370
CGAGCGG24300.020.10884770
CGTCTAG17200.018.8875521
AGCGATG21050.018.70443370
CCGTATC11200.017.49552749
CTGCCGG39200.017.4874870
CGATCTA82200.017.32055770
GCGATCG9200.016.81293570
AGCGATC150500.016.79302469
CGCCGTA12650.016.3156647
TCGCCGT12950.015.66866646
CGGTTCG7150.015.1106212
TACGGTG12800.014.96914514
CGTATCA13850.014.95262962
CTACGGT12350.014.95189613
GAGCGAT196750.014.47127369
CGGCTAT21150.014.0665935
AGCGGTT13550.013.67793710
CCTACGG13350.013.57527812
Produced by FastQC (version 0.11.5)