FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22906007
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC19750.040.93740570
GCCGACC31300.032.4286770
CGATCTA103500.022.75884270
CGTCTAG26700.022.6778951
GCGATCT151400.021.17605270
TTGACGG30100.018.60460331
CTACGGT23800.018.23524713
TAGACGC43700.017.2997264
CTAGACG25000.017.2199553
GACGCAC26100.016.8965076
TGACGGC33450.016.84599532
TCCGCAA24750.016.82823833
ACGCACC26050.016.794587
TACGGTG25850.016.78912414
AGCGATA11650.016.52388670
AGCGATC200850.016.52007169
CGCCGTA5350.016.3552459
GACGAGC494400.015.80124170
CCTACGG28300.015.58299812
GACGGCT36650.015.47062933
Produced by FastQC (version 0.11.5)