FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28251737
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC22600.032.0584770
GCCGACC37000.026.48727470
CGTCTAG31200.025.9134061
CGCCGTA7350.024.28583359
GCGATCT345450.023.06046170
CGATCTA229200.022.72318670
GCGATCA14400.020.90339770
CTACGGT27050.020.31415413
AGCGATA21150.019.03129870
TTGACGG38100.018.9238131
CTAGACG31950.018.4036853
TACGGTG30050.018.28611814
AGCGATC466250.017.68631669
TAGACGC54250.017.4192854
TCCGCAA31000.017.38702833
CCTACGG33000.016.7575112
TGACGGC43650.016.59787432
GTCCGCA33400.016.4520332
CGTATCA11700.016.15395562
GACGCAC35000.015.9999376
Produced by FastQC (version 0.11.5)