FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26425651
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG746810.2826079857029823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA9750.033.38468659
CGTATCA11700.028.71800862
CGACGAC17550.027.72127570
CCGTATC13750.024.6909661
CGTCTAG32450.023.7287731
TCGCCGT14200.023.16906258
GCGATCT266200.022.75959470
CGATCTA177050.022.43755170
CTACGGT31300.020.79868513
TACGGTG31150.020.67412214
CTAGACG33250.020.3157143
GACGCAC36150.018.7827826
TTGACGG38300.018.6422331
ACGCACC35550.018.6075277
CCTACGG35700.018.52937912
TCCGCAA35050.018.37368433
GTCCGCA35800.017.7932332
AGCGATC353400.017.51017269
TAGACGC58900.017.4702264
ACGGTGA38300.017.36289215
Produced by FastQC (version 0.11.5)